Upgrade to nextclade v3 & update default dataset tags (#375)

* added new WDL task for nextclade v3. tested w miniwdl. not added to workflows yet * added common miniwdl output directories to .gitignore * update sars-cov-2 nextclade defaults; removed unnecessary nextclade_dataset_reference inputs and outputs from organism_param subworkflow * updates to nextclade v3 task * update theiacov_fasta to use nextclade v3 task. tested successfully with miniwdl and sc2 sample * update nextclade defaults for non-sc2 organisms. Have not tested at all, need to test in Terra * update to nextclade 3.3.1 and implement --verbosity flag for nextclade commands * updated WDL task for adding samples to nextclade ref tree. tested fine with miniwdl and a Flu HA FASTA * update Sample_to_ref_tree_PHB workflow: removed old inputs and made assembly_fasta input singular * updated theiacov_fasta_batch, ilmn pe, ilmn se, and ont to use nextclade v3. need to test on Terra still (have not tested with miniwdl) * update theiacov_clearlabs to use nextclade_v3. did not test with miniwdl so will need to test in Terra * fix import path for organism_paramteters subwf in theiacov_clearlabs workflow * shellcheck lied to me. reverting last commit * update theiacov_fasta CI * update theiacov_clearlabs CI * update theiacov_ont CI * re-enable theiacov_illumina_pe and se CI workflows; update them for nextclade_v3 and a slew of other updates from previous PRs that were missed since the CI was disabled * update CI * nextclade_v3 task: removed unused pcr_primers_csv input; added back input_ref optional input. tested successfully with miniwdl * nextclade_addToRefTree task and wf change: remove input-pcr-primers option and add input-ref option * corrected input file type for input-ref
theiagen · Apr 4, 2024 · 51274c7 · 51274c7
1 parent c0925a8
commit 51274c7
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diff --git a/.gitignore b/.gitignore
@@ -1 +1,3 @@
-cromwell*
+cromwell*
+_LAST
+2024*
diff --git a/tasks/taxon_id/task_nextclade.wdl b/tasks/taxon_id/task_nextclade.wdl
@@ -60,6 +60,72 @@ task nextclade {
   }
 }
 
+task nextclade_v3 {
+  meta {
+    description: "Nextclade classification of one sample. Leaving optional inputs unspecified will use SARS-CoV-2 defaults."
+  }
+  input {
+    File genome_fasta
+    File? auspice_reference_tree_json
+    File? gene_annotations_gff
+    File? nextclade_pathogen_json
+    File? input_ref
+    String docker = "us-docker.pkg.dev/general-theiagen/nextstrain/nextclade:3.3.1" 
+    String dataset_name
+    String verbosity = "warn" # other options are: "off" "error" "info" "debug" and "trace"
+    String dataset_tag
+    Int disk_size = 50
+    Int memory = 4
+    Int cpu = 2
+  }
+  String basename = basename(genome_fasta, ".fasta")
+  command <<<
+    # track version & print to log
+    nextclade --version | tee NEXTCLADE_VERSION
+
+    # --reference no longer used in v3. consolidated into --name and --tag
+    nextclade dataset get \
+      --name="~{dataset_name}" \
+      --tag="~{dataset_tag}" \
+      -o nextclade_dataset_dir \
+      --verbosity ~{verbosity}
+
+    # exit script/task upon error
+    set -e
+
+    # not necessary to include `--jobs <jobs>` in v3. Nextclade will use all available CPU threads by default. It's fast so I don't think we will need to change unless we see errors
+    nextclade run \
+      --input-dataset nextclade_dataset_dir/ \
+      ~{"--input-ref " + input_ref} \
+      ~{"--input-tree " + auspice_reference_tree_json} \
+      ~{"--input-pathogen-json " + nextclade_pathogen_json} \
+      ~{"--input-annotation " + gene_annotations_gff} \
+      --output-json "~{basename}".nextclade.json \
+      --output-tsv  "~{basename}".nextclade.tsv \
+      --output-tree "~{basename}".nextclade.auspice.json \
+      --output-all . \
+      --verbosity ~{verbosity} \
+      "~{genome_fasta}"
+  >>>
+  runtime {
+    docker: "~{docker}"
+    memory: "~{memory} GB"
+    cpu: cpu
+    disks:  "local-disk " + disk_size + " SSD"
+    disk: disk_size + " GB" # TES
+    dx_instance_type: "mem1_ssd1_v2_x2"
+    maxRetries: 3 
+  }
+  output {
+    String nextclade_version = read_string("NEXTCLADE_VERSION")
+    File nextclade_json = "~{basename}.nextclade.json"
+    File auspice_json = "~{basename}.nextclade.auspice.json"
+    File nextclade_tsv = "~{basename}.nextclade.tsv"
+    String nextclade_docker = docker
+    String nextclade_dataset_tag = "~{dataset_tag}"
+  }
+}
+
 task nextclade_output_parser {
   meta {
     description: "Python and bash codeblocks for parsing the output files from Nextclade."
@@ -163,52 +229,49 @@ task nextclade_add_ref {
   }
   input {
     File genome_fasta
-    File? root_sequence
     File? reference_tree_json
-    File? qc_config_json
+    File? nextclade_pathogen_json
     File? gene_annotations_gff
-    File? pcr_primers_csv
-    File? virus_properties
-    String docker = "us-docker.pkg.dev/general-theiagen/nextstrain/nextclade:2.14.0"
+    File? input_ref
+    String docker = "us-docker.pkg.dev/general-theiagen/nextstrain/nextclade:3.3.1"
     String dataset_name
-    String? dataset_reference
     String? dataset_tag
-    Int disk_size = 50
-    Int memory = 8
+    String verbosity = "warn" # other options are: "off" "error" "info" "debug" and "trace"
+    Int disk_size = 100
+    Int memory = 4
     Int cpu = 2
   }
   String basename = basename(genome_fasta, ".fasta")
   command <<<
-    NEXTCLADE_VERSION="$(nextclade --version)"
-    echo $NEXTCLADE_VERSION > NEXTCLADE_VERSION
+    # track version & print to log
+    nextclade --version | tee NEXTCLADE_VERSION
 
+    echo "DEBUG: downloading nextclade dataset..."
     nextclade dataset get \
       --name="~{dataset_name}" \
-      ~{"--reference " + dataset_reference} \
       ~{"--tag " + dataset_tag} \
       -o nextclade_dataset_dir \
-      --verbose
+      --verbosity ~{verbosity}
 
-    # If no referece sequence is provided, use the reference tree from the dataset
+    # If no reference sequence is provided, use the reference tree from the dataset
     if [ -z "~{reference_tree_json}" ]; then
       echo "Default dataset reference tree JSON will be used"
-      cp nextclade_dataset_dir/tree.json reference_tree.json
+      cp -v nextclade_dataset_dir/tree.json reference_tree.json
     else
       echo "User reference tree JSON will be used"
-      cp ~{reference_tree_json} reference_tree.json
+      cp -v ~{reference_tree_json} reference_tree.json
     fi
 
     tree_json="reference_tree.json"
 
     set -e
+    echo "DEBUG: running nextclade..."
     nextclade run \
-      --input-dataset=nextclade_dataset_dir/ \
-      ~{"--input-root-seq " + root_sequence} \
+      --input-dataset nextclade_dataset_dir/ \
       --input-tree ${tree_json} \
-      ~{"--input-qc-config " + qc_config_json} \
-      ~{"--input-gene-map " + gene_annotations_gff} \
-      ~{"--input-pcr-primers " + pcr_primers_csv} \
-      ~{"--input-virus-properties " + virus_properties}  \
+      ~{"--input-pathogen-json " + nextclade_pathogen_json} \
+      ~{"--input-annotation " + gene_annotations_gff} \
+      ~{"--input-ref " + input_ref} \
       --output-json "~{basename}".nextclade.json \
       --output-tsv  "~{basename}".nextclade.tsv \
       --output-tree "~{basename}".nextclade.auspice.json \

diff --git a/tests/workflows/theiacov/test_wf_theiacov_clearlabs.yml b/tests/workflows/theiacov/test_wf_theiacov_clearlabs.yml
@@ -212,67 +212,58 @@
     - path: miniwdl_run/call-ncbi_scrub_se/work/clearlabs_R1_dehosted.fastq.gz
     - path: miniwdl_run/call-ncbi_scrub_se/work/r1.fastq
     - path: miniwdl_run/call-ncbi_scrub_se/work/r1.fastq.clean
-    - path: miniwdl_run/call-nextclade/command
-    - path: miniwdl_run/call-nextclade/inputs.json
+    - path: miniwdl_run/call-nextclade_v3/command
+    - path: miniwdl_run/call-nextclade_v3/inputs.json
       contains: ["dataset_name", "dataset_tag", "genome_fasta"]
-    - path: miniwdl_run/call-nextclade/outputs.json
+    - path: miniwdl_run/call-nextclade_v3/outputs.json
       contains: ["nextclade", "nextclade_json", "nextclade_version"]
-    - path: miniwdl_run/call-nextclade/stderr.txt
-    - path: miniwdl_run/call-nextclade/stderr.txt.offset
-    - path: miniwdl_run/call-nextclade/stdout.txt
-    - path: miniwdl_run/call-nextclade/task.log
+    - path: miniwdl_run/call-nextclade_v3/stderr.txt
+    - path: miniwdl_run/call-nextclade_v3/stderr.txt.offset
+    - path: miniwdl_run/call-nextclade_v3/stdout.txt
+    - path: miniwdl_run/call-nextclade_v3/task.log
       contains: ["wdl", "theiacov_clearlabs", "nextclade", "done"]
-    - path: miniwdl_run/call-nextclade/work/NEXTCLADE_VERSION
-      md5sum: 91a455762183b41af0d8de5596e28e7f
-    - path: miniwdl_run/call-nextclade/work/_miniwdl_inputs/0/clearlabs.medaka.consensus.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/NEXTCLADE_VERSION
+      md5sum: 70aa6879bf9f0e8ba2b9953b0d4a2216
+    - path: miniwdl_run/call-nextclade_v3/work/_miniwdl_inputs/0/clearlabs.medaka.consensus.fasta
       md5sum: d41d8cd98f00b204e9800998ecf8427e
-    - path: miniwdl_run/call-nextclade/work/clearlabs.medaka.consensus.nextclade.auspice.json
-    - path: miniwdl_run/call-nextclade/work/clearlabs.medaka.consensus.nextclade.json
-    - path: miniwdl_run/call-nextclade/work/clearlabs.medaka.consensus.nextclade.tsv
-    - path: miniwdl_run/call-nextclade/work/nextclade.aligned.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.medaka.consensus.nextclade.auspice.json
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.medaka.consensus.nextclade.json
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.medaka.consensus.nextclade.tsv
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.aligned.fasta
       md5sum: eb18c508f26125851279f2c03d4a336c
-    - path: miniwdl_run/call-nextclade/work/nextclade.csv
-    - path: miniwdl_run/call-nextclade/work/nextclade.errors.csv
-      md5sum: 2d1dad70d68e56d0a1191900c17061bc
-    - path: miniwdl_run/call-nextclade/work/nextclade.insertions.csv
-      md5sum: 3fb6db0807dc663e2821e0bbbccdc5aa
-    - path: miniwdl_run/call-nextclade/work/nextclade.ndjson
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/genemap.gff
-      md5sum: b4bd70a3779718e556a17360a41dce90
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/primers.csv
-      md5sum: 5990c3483bf66ce607aeb90a44e7ef2e
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/qc.json
-      md5sum: b01f4491a54941fea12ec5b04a10fb8c
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/reference.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.csv
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.ndjson
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/genome_annotation.gff3
+      md5sum: 4dff84d2d6ada820e0e3a8bc6798d402
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/pathogen.json
+      md5sum: 9f99ba19333ff907af307611fbb73e21
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/reference.fasta
       md5sum: c7ce05f28e4ec0322c96f24e064ef55c
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/sequences.fasta
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/tag.json
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/tree.json
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/virus_properties.json
-      md5sum: 03bd2f9d33326299b5b49b4910d84183
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_E.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/sequences.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/tree.json
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.E.fasta
       md5sum: 14808ad8b34c8bac7de500707400250e
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_M.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.M.fasta
       md5sum: 4799e5af880d2005da56342d6a9d64ab
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_N.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.N.fasta
       md5sum: bbc46cedb153b3213a9cf8f425dd906c
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF1a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF1a.fasta
       md5sum: 0c1b1bbcbcfe86d10c466bf63fca5c11
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF1b.translation.fasta
-      md5sum: 23a0497efe0ccffaf51b792f40ca5036
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF3a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF1b.fasta
+      md5sum: bea75a83074a11fa74c316e4df6a3d9f
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF3a.fasta
       md5sum: 692b2c314c4ff6584a40273dc239cb78
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF6.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF6.fasta
       md5sum: c1d610f9e45acd3915e40f0d643f0188
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF7a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF7a.fasta
       md5sum: a655a6c325b0bc9ad69842fcf2e927a7
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF7b.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF7b.fasta
       md5sum: 27fd219bb6d18731898a9ddfdee27f67
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF8.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF8.fasta
       md5sum: 398798980c482562e7c5b21b205e0445
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF9b.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF9b.fasta
       md5sum: 3d6a949bdcecaf70e9d123651a7a7c5e
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_S.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.S.fasta
       md5sum: 0ce44a0a8e2784ca4b3e8d8f03211813
     - path: miniwdl_run/call-nextclade_output_parser/command
       md5sum: f377fb9fc901d440fa35b1b05317a0e1

diff --git a/tests/workflows/theiacov/test_wf_theiacov_fasta.yml b/tests/workflows/theiacov/test_wf_theiacov_fasta.yml
@@ -36,67 +36,59 @@
     - path: miniwdl_run/call-consensus_qc/work/PERCENT_REF_COVERAGE
       md5sum: 6808ca805661622ad65ae014a4b2a094
     - path: miniwdl_run/call-consensus_qc/work/_miniwdl_inputs/0/clearlabs.fasta.gz
-    - path: miniwdl_run/call-nextclade/command
-      md5sum: 5574a2eac479f797176a8ecc305b5e0d
-    - path: miniwdl_run/call-nextclade/inputs.json
-    - path: miniwdl_run/call-nextclade/outputs.json
-    - path: miniwdl_run/call-nextclade/stderr.txt
-    - path: miniwdl_run/call-nextclade/stderr.txt.offset
-    - path: miniwdl_run/call-nextclade/stdout.txt
-    - path: miniwdl_run/call-nextclade/task.log
-    - path: miniwdl_run/call-nextclade/work/NEXTCLADE_VERSION
-      md5sum: 91a455762183b41af0d8de5596e28e7f
-    - path: miniwdl_run/call-nextclade/work/_miniwdl_inputs/0/clearlabs.fasta.gz
-    - path: miniwdl_run/call-nextclade/work/clearlabs.fasta.gz.nextclade.auspice.json
-    - path: miniwdl_run/call-nextclade/work/clearlabs.fasta.gz.nextclade.json
-    - path: miniwdl_run/call-nextclade/work/clearlabs.fasta.gz.nextclade.tsv
-      md5sum: 9b459df2831ab1974c9c8cef5bc5340e
-    - path: miniwdl_run/call-nextclade/work/nextclade.aligned.fasta
+    - path: miniwdl_run/call-nextclade_v3/command
+      md5sum: 2cd5aabef6c3aaa89a503e59a3b6b36c
+    - path: miniwdl_run/call-nextclade_v3/inputs.json
+    - path: miniwdl_run/call-nextclade_v3/outputs.json
+    - path: miniwdl_run/call-nextclade_v3/stderr.txt
+    - path: miniwdl_run/call-nextclade_v3/stderr.txt.offset
+    - path: miniwdl_run/call-nextclade_v3/stdout.txt
+    - path: miniwdl_run/call-nextclade_v3/task.log
+    - path: miniwdl_run/call-nextclade_v3/work/NEXTCLADE_VERSION
+      md5sum: 70aa6879bf9f0e8ba2b9953b0d4a2216
+    - path: miniwdl_run/call-nextclade_v3/work/_miniwdl_inputs/0/clearlabs.fasta.gz
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.fasta.gz.nextclade.auspice.json
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.fasta.gz.nextclade.json
+    - path: miniwdl_run/call-nextclade_v3/work/clearlabs.fasta.gz.nextclade.tsv
+      md5sum: 3aeae954ba64b8ad7db55e08f9c7131c
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.aligned.fasta
       md5sum: bf487271d506418ea23fe30fc033e44d
-    - path: miniwdl_run/call-nextclade/work/nextclade.csv
-      md5sum: 2993ec6e17c617b665d0662488e2d657
-    - path: miniwdl_run/call-nextclade/work/nextclade.errors.csv
-      md5sum: 483415f75e782897d83fc1305f793e21
-    - path: miniwdl_run/call-nextclade/work/nextclade.insertions.csv
-      md5sum: eb3f8ec90ecd728a0b03a14171085702
-    - path: miniwdl_run/call-nextclade/work/nextclade.ndjson
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/genemap.gff
-      md5sum: b4bd70a3779718e556a17360a41dce90
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/primers.csv
-      md5sum: 5990c3483bf66ce607aeb90a44e7ef2e
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/qc.json
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/reference.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.csv
+      md5sum: 50ca5404982b62cbdf077c5d16543e6f
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.ndjson
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/genome_annotation.gff3
+      md5sum: 4dff84d2d6ada820e0e3a8bc6798d402
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/pathogen.json
+      md5sum: 9f99ba19333ff907af307611fbb73e21
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/reference.fasta
       md5sum: c7ce05f28e4ec0322c96f24e064ef55c
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/sequences.fasta
-      md5sum: ea475ab0a62a0a68fc3b1108fdff8a20
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/tag.json
-      md5sum: 7c080257b26eb528c72858e369fec68c
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/tree.json
-      md5sum: 6b227b0aeeda07a187f5879e810879a3
-    - path: miniwdl_run/call-nextclade/work/nextclade_dataset_dir/virus_properties.json
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_E.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/sequences.fasta
+      md5sum: c2a4d6cbb837dce22d81f9c36dd0629e
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade_dataset_dir/tree.json
+      md5sum: ae2f621cbd025d28389282ed5403fedc
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.E.fasta
       md5sum: dc43b1e98245a25c142aec52b29a07df
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_M.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.M.fasta
       md5sum: 440de8feeb02bd06878c63319513fb74
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_N.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.N.fasta
       md5sum: 8a45a3246eb19643cb305a29a3f7f712
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF1a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF1a.fasta
       md5sum: b2f11d3bc5674501f9231bfe37e87412
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF1b.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF1b.fasta
       md5sum: 7597678bc323068f31cd6cc726384903
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF3a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF3a.fasta
       md5sum: 13a0d63ae18a7975413b54a97cfa0dd5
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF6.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF6.fasta
       md5sum: ae20a4dae100edd4033cb8af32a4d0bc
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF7a.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF7a.fasta
       md5sum: 31d03e19799bc52da442217a5e1f1313
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF7b.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF7b.fasta
       md5sum: 5a505858730e58ac19c052ea2ee84517
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF8.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF8.fasta
       md5sum: 0e8b98d96c0d75386f08755ea9b3899d
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_ORF9b.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.ORF9b.fasta
       md5sum: fb478d9f262d6a4b3ca5554e4b6654b9
-    - path: miniwdl_run/call-nextclade/work/nextclade_gene_S.translation.fasta
+    - path: miniwdl_run/call-nextclade_v3/work/nextclade.cds_translation.S.fasta
       md5sum: e630a638abbb2c8ab4a8b74455e9668f
     - path: miniwdl_run/call-nextclade_output_parser/command
       md5sum: 0805cf58414b2ffaf3ea5e53f85a5f3d
@@ -119,7 +111,7 @@
     - path: miniwdl_run/call-nextclade_output_parser/work/_miniwdl_inputs/0/clearlabs.fasta.gz.nextclade.tsv
       md5sum: d41d8cd98f00b204e9800998ecf8427e
     - path: miniwdl_run/call-nextclade_output_parser/work/input.tsv
-      md5sum: 9b459df2831ab1974c9c8cef5bc5340e
+      md5sum: 3aeae954ba64b8ad7db55e08f9c7131c
     - path: miniwdl_run/call-pangolin4/command
       md5sum: b9c36681b77c5e007bf7e890265d70eb
     - path: miniwdl_run/call-pangolin4/inputs.json