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Twinkle

This repository documents a microscope for Two-photon Imaging in Neuroscience, and Kit for Learning and Education — "Twinkle".
It offers an affordable solution for a fully open, high-performance and cost-effective research and teaching microscope. Here, you can find the complete design and documentation.

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Repository Content

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├── article                 # Latex code and figures for the main manuscript.
├── assets                  # Supporting files, OSHW certification.
├── cad                     # Computer-aided design (CAD) files of the entire instrument in various file formats.
├── documentation           # Build instructions and supplemental material.
├── parts                   # Detailed bill of materials (BoM), important quotes, and drawings for machining.
    ├── Drawings            # Drawings for machining.
    ├── various files       # BoM and important quotes.
├── zemax                   # Optics simulations.
    ├── Collection          # Zemax files, drawings, and simulated performance of the collection optics.
    ├── Excitation          # Zemax files, drawings, and simulated performance of the excitation optics.
    ├── matlab              # Matlab scripts, using the Zemax API, measuring the performance of the excitation system.
├── LICENSE                 # Text file: This work is licensed under a CC Attribution 4.0 International License.
├── README.md               # Text file: This info file.
└── okh-manifest.yml        # Text file: Open Know-How manifest files.   

Computer assisted design (CAD) files

The CAD files are provided in two formats:

  • In the Fusion 360 native format as FullAssembly.f3d; md5 hash cbc14d6bce3a4f311935500c3b959128.
  • For Autodesk Inventor:
    1. When using the Inventor folder, open the master assembly FullAssembly.iam.
    2. A zip file of the same data is split into three files. Assemble the data with cat x?? > FullAssembly.zip. The file FullAssembly.zip is 166 MB with md5 hash 130a4bcaa6f747f43ba337dc33d74409.

Issue

Issues are tracked through this repository. For minor updates, just push. For major ones, use a PR.

License

This work is free: you can redistribute it and/or modify it under the terms of the Creative Commons Attribution 4.0 International license, version 4 of the License, or (at your option) any later version (CC-BY-4.0). This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY, to the extent permitted by law; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. A copy of the License is provided in this repository. For more details, see http://www.gnu.org/licenses/.

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Design and documentation for the 2p microscope.

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