This repository provides scripts for the phylogenetic analysis of the CCHF virus.
To run the workflow using TreeKnit you need to set up an environment containing Nextstrain and Julia with TreeKnit and TreeTools. The workflow can then be build from within this environment.
Download the raw data of CCHFV sequences e.g. from the NCBI Virus database.
For filtering the data the python script scripts/filter_rawdata.py can be used. This will create separate fasta files and metadata files for each segment of the CCHF virus in the data folder, which are needed as input for the workflow.
There are three different snakefile versions provided within this repository.
The standard snakefile called Snakefile contains the whole workflow including a resolving step using TreeKnit. To use TreeKnit it is necessary to call a Julia script and thus Julia has to be installed in the environment. The workflow could be run e.g. within a micromamba environment containing Nextstrain, Julia and TreeKnit with the command:
nextstrain build .
If julia is not available or TreeKnit should be excluded from the workflow the snakefile Snakefile_without_treeknit can be used alternatively, by using the file option of snakemake:
-s FILE
The third snakefile Snakefile_without_linking creates the phylogenetic trees of all segments separately without ensuring that the sequences for each segment are chosen from the same set of samples, making it harder to create tanglegrams of the trees.
For local development activate the micromamba environment using:
micromamba create -f environment.yml --platform=linux/amd64
You can format your snakemake files using
snakefmt ${file}
You can visualize the workflow using:
snakemake -s ${file} --dag | dot -Tpng > dag.png