Python/C++ port of the ImageJ extension TurboReg/StackReg written by Philippe Thevenaz/EPFL.
A python extension for the automatic alignment of a source image or a stack (movie) to a target image/reference frame.
pyStackReg is used to align (register) one or more images to a common reference image, as is required usually in time-resolved fluorescence or wide-field microscopy. It is directly ported from the source code of the ImageJ plugin TurboReg and provides additionally the functionality of the ImageJ plugin StackReg, both of which were written by Philippe Thevenaz/EPFL (available at http://bigwww.epfl.ch/thevenaz/turboreg/).
pyStackReg provides the following five types of distortion:
- translation
- rigid body (translation + rotation)
- scaled rotation (translation + rotation + scaling)
- affine (translation + rotation + scaling + shearing)
- bilinear (non-linear transformation; does not preserve straight lines)
pyStackReg supports the full functionality of StackReg plus some additional options, e.g., using different reference images and having access to the actual transformation matrices (please see the examples below). Note that pyStackReg uses the high quality (i.e. high accuracy) mode of TurboReg that uses cubic spline interpolation for transformation.
Please note: The bilinear transformation cannot be propagated, as a combination of bilinear transformations does not generally result in a bilinear transformation. Therefore, stack registration/transform functions won't work with bilinear transformation when using "previous" image as reference image. You can either use another reference ("first" or "mean" for first or mean image, respectively), or try to register/transform each image of the stack separately to its respective previous image (and use the already transformed previous image as reference for the next image).
- pystackreg (and StackReg/TurboReg) have known issues with processing strongly rotated images (e.g. by ~90°; see #30 for a workaround).
The package is available on conda forge and on PyPi.
- Install using conda
conda install pystackreg -c conda-forge- Install using pip
pip install pystackregThe documentation can be found on readthedocs:
https://pystackreg.readthedocs.io/
- A tutorial notebook can be found in the examples/notebooks folder or statically here: https://pystackreg.readthedocs.io/en/latest/tutorial.html
The following example opens two different files and registers them using all different possible transformations
from pystackreg import StackReg
from skimage import io
#load reference and "moved" image
ref = io.imread('some_original_image.tif')
mov = io.imread('some_changed_image.tif')
#Translational transformation
sr = StackReg(StackReg.TRANSLATION)
out_tra = sr.register_transform(ref, mov)
#Rigid Body transformation
sr = StackReg(StackReg.RIGID_BODY)
out_rot = sr.register_transform(ref, mov)
#Scaled Rotation transformation
sr = StackReg(StackReg.SCALED_ROTATION)
out_sca = sr.register_transform(ref, mov)
#Affine transformation
sr = StackReg(StackReg.AFFINE)
out_aff = sr.register_transform(ref, mov)
#Bilinear transformation
sr = StackReg(StackReg.BILINEAR)
out_bil = sr.register_transform(ref, mov)The next example shows how to separate registration from transformation (e.g., to register in one color channel and then use that information to transform another color channel):
from pystackreg import StackReg
from skimage import io
img0 = io.imread('some_multiframe_image.tif')
img1 = io.imread('another_multiframe_image.tif')
# img0.shape: frames x width x height (3D)
sr = StackReg(StackReg.RIGID_BODY)
# register 2nd image to 1st
sr.register(img0[0, :, :], img0[1,:,:])
# use the transformation from the above registration to register another frame
out = sr.transform(img1[1,:,:])The next examples shows how to register and transform a whole stack:
from pystackreg import StackReg
from skimage import io
img0 = io.imread('some_multiframe_image.tif') # 3 dimensions : frames x width x height
sr = StackReg(StackReg.RIGID_BODY)
# register each frame to the previous (already registered) one
# this is what the original StackReg ImageJ plugin uses
out_previous = sr.register_transform_stack(img0, reference='previous')
# register to first image
out_first = sr.register_transform_stack(img0, reference='first')
# register to mean image
out_mean = sr.register_transform_stack(img0, reference='mean')
# register to mean of first 10 images
out_first10 = sr.register_transform_stack(img0, reference='first', n_frames=10)
# calculate a moving average of 10 images, then register the moving average to the mean of
# the first 10 images and transform the original image (not the moving average)
out_moving10 = sr.register_transform_stack(img0, reference='first', n_frames=10, moving_average = 10)The next example shows how to separate registration from transformation for a stack (e.g., to register in one color channel and then use that information to transform another color channel):
from pystackreg import StackReg
from skimage import io
img0 = io.imread('some_multiframe_image.tif') # 3 dimensions : frames x width x height
img1 = io.imread('another_multiframe_image.tif') # same shape as img0
# both stacks must have the same shape
assert img0.shape == img1.shape
sr = StackReg(StackReg.RIGID_BODY)
# register each frame to the previous (already registered) one
# this is what the original StackReg ImageJ plugin uses
tmats = sr.register_stack(img0, reference='previous')
out = sr.transform_stack(img1)
# tmats contains the transformation matrices -> they can be saved
# and loaded at another time
import numpy as np
np.save('transformation_matrices.npy', tmats)
tmats_loaded = np.load('transformation_matrices.npy')
# make sure you use the correct transformation here!
sr = StackReg(StackReg.RIGID_BODY)
# transform stack using the tmats loaded from file
sr.transform_stack(img1, tmats=tmats_loaded)
# with the transformation matrices at hand you can also
# use the transformation algorithms from other packages:
from skimage import transform as tf
out = np.zeros(img0.shape).astype(np.float)
for i in range(tmats.shape[0]):
out[i, :, :] = tf.warp(img1[i, :, :], tmats[i, :, :], order=3)This is a port of the original Java code by Philippe Thevenaz to C++ with a Python wrapper around it. All credit goes to the original author:
/*==================================================================== | Philippe Thevenaz | EPFL/STI/IMT/LIB/BM.4.137 | Station 17 | CH-1015 Lausanne VD | Switzerland | | phone (CET): +41(21)693.51.61 | fax: +41(21)693.37.01 | RFC-822: [email protected] | X-400: /C=ch/A=400net/P=switch/O=epfl/S=thevenaz/G=philippe/ | URL: http://bigwww.epfl.ch/ \===================================================================*/ /*==================================================================== | This work is based on the following paper: | | P. Thevenaz, U.E. Ruttimann, M. Unser | A Pyramid Approach to Subpixel Registration Based on Intensity | IEEE Transactions on Image Processing | vol. 7, no. 1, pp. 27-41, January 1998. | | This paper is available on-line at | http://bigwww.epfl.ch/publications/thevenaz9801.html | | Other relevant on-line publications are available at | http://bigwww.epfl.ch/publications/ \===================================================================*/
You are free to use this software for commercial and non-commercial purposes. However, we expect you to include a citation or acknowledgement whenever you present or publish research results that are based on this software. You are free to modify this software or derive works from it, but you are only allowed to distribute it under the same terms as this license specifies. Additionally, you must include a reference to the research paper above in all software and works derived from this software.