Experimental protocols and bioinformatic pipelines for Zika genome sequencing

Allison Black^1,2, Barney Potter², Josh Quick³, Nicholas J. Loman³, Nathan D Grubaugh⁴, Kristian G Andersen⁴, Trevor Bedford²

¹Department of Epidemiology, University of Washington, Seattle, WA, USA, ²Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA, ³Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, UK, ⁴The Scripps Research Institute, La Jolla, CA, USA.

• Sample metadata
• Experimental protocols
• Bioinformatic pipelines
• Validation and coverage
• Consensus genomes