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I am using the installation instructions provided on the homepage to install bcbio-vm. I have completed the setup till adding the docker group. I am not able to install the docker image, the genome data and the tools. Looks like the download is fine, but something goes wrong during installation.
The log:
bcbio_vm.py --datadir=~/install/bcbio-vm/data install --data --tools --genomes GRCh37 --aligners bwa
Retrieving bcbio-nextgen docker image with code and tools
Using default tag: latest
latest: Pulling from bcbio/bcbio-vc
Digest: sha256:97bcadba81529438ecb3ffb703211f0b6a80d9e252512312d684d68bbda45b75
Status: Image is up to date for quay.io/bcbio/bcbio-vc:latest
quay.io/bcbio/bcbio-vc:latest
[2020-05-27T06:45Z] DEBUG: Running in docker container: 870fa26ee434a6522f800818 ec33cf2a7804f6c9dc502fd719ce2c43c3221d46
[2020-05-27T06:45Z] DEBUG: docker attach --no-stdin 870fa26ee434a6522f800818ec33 cf2a7804f6c9dc502fd719ce2c43c3221d46
[2020-05-27T06:45Z] DEBUG: Upgrading bcbio
[2020-05-27T06:45Z] DEBUG: Upgrade completed successfully.
[2020-05-27T06:45Z] DEBUG: Running in docker container: 12d33b4f2f096c192aad42a7 73fdad0abe0b6d86e71ec75b057939918ec41691
[2020-05-27T06:45Z] DEBUG: docker attach --no-stdin 12d33b4f2f096c192aad42a773fd ad0abe0b6d86e71ec75b057939918ec41691
[2020-05-27T06:45Z] DEBUG: --2020-05-27 06:45:28-- https://github.com/chapmanb/ cloudbiolinux/archive/master.tar.gz
[2020-05-27T06:45Z] DEBUG: Resolving github.com (github.com)... 13.234.210.38
[2020-05-27T06:45Z] DEBUG: Connecting to github.com (github.com)|13.234.210.38|: 443... connected.
[2020-05-27T06:45Z] DEBUG: HTTP request sent, awaiting response... 302 Found
[2020-05-27T06:45Z] DEBUG: Location: https://codeload.github.com/chapmanb/cloudb iolinux/tar.gz/master [following]
[2020-05-27T06:45Z] DEBUG: --2020-05-27 06:45:28-- https://codeload.github.com/ chapmanb/cloudbiolinux/tar.gz/master
[2020-05-27T06:45Z] DEBUG: Resolving codeload.github.com (codeload.github.com).. . 13.127.152.42
[2020-05-27T06:45Z] DEBUG: Connecting to codeload.github.com (codeload.github.co m)|13.127.152.42|:443... connected.
[2020-05-27T06:45Z] DEBUG: HTTP request sent, awaiting response... 200 OK
[2020-05-27T06:45Z] DEBUG: Length: unspecified [application/x-gzip]
[2020-05-27T06:45Z] DEBUG: Saving to: 'STDOUT'
[2020-05-27T06:45Z] DEBUG: 0K ........ ........ ........ ........ ........ ........ 6.95M
[2020-05-27T06:45Z] DEBUG: 3072K ........ ........ ........ ....... 7.06M=0.7s
[2020-05-27T06:45Z] DEBUG: 2020-05-27 06:45:30 (6.99 MB/s) - written to stdout [ 5189878]
[2020-05-27T06:45Z] DEBUG: --2020-05-27 06:45:30-- http://ftp.ncbi.nih.gov/snp/ pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
[2020-05-27T06:45Z] DEBUG: Resolving ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)... 130.14.250.7, 2607:f220:41e:250::11
[2020-05-27T06:45Z] DEBUG: Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:80... connected.
[2020-05-27T06:45Z] DEBUG: HTTP request sent, awaiting response... 301 Moved Permanently
[2020-05-27T06:45Z] DEBUG: Location: https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz [following]
[2020-05-27T06:45Z] DEBUG: --2020-05-27 06:45:35-- https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
[2020-05-27T06:45Z] DEBUG: Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:443... connected.
[2020-05-27T06:45Z] DEBUG: HTTP request sent, awaiting response... 416 Requested Range Not Satisfiable
[2020-05-27T06:45Z] DEBUG: The file is already fully retrieved; nothing to do.
[2020-05-27T07:00Z] DEBUG: [W::bgzf_read_block] EOF marker is absent. The input is probably truncated
[2020-05-27T07:00Z] DEBUG: tbx_index_build failed: variation/dbsnp-151.vcf.gz
[2020-05-27T07:00Z] DEBUG: Upgrading bcbio
[2020-05-27T07:00Z] DEBUG: Upgrading bcbio-nextgen data files
[2020-05-27T07:00Z] DEBUG: List of genomes to get (from the config file at '{'genomes': [{'dbkey': 'GRCh37', 'name': 'Human (GRCh37)', 'indexes': ['seq', 'twobit'], 'annotations': ['GA4GH_problem_regions', 'capture_regions', 'MIG', 'prioritize', 'dbsnp', 'hapmap', '1000g_omni_snps', 'ACMG56_genes', '1000g_snps', 'mills_indels', 'clinvar', 'cosmic', 'ancestral', 'qsignature', 'genesplicer', 'effects_transcripts', 'varpon', 'vcfanno', 'viral', 'transcripts', 'RADAR', 'fusion-blacklist', 'mirbase'], 'validation': ['giab-NA12878', 'giab-NA24385', 'giab-NA24631', 'dream-syn3', 'dream-syn4', 'giab-NA12878-NA24385-somatic', 'giab-NA24143', 'giab-NA24149', 'giab-NA24694', 'giab-NA24695']}], 'genome_indexes': ['bwa', 'rtg'], 'install_liftover': False, 'install_uniref': False}'): Human (GRCh37)
[2020-05-27T07:00Z] DEBUG: Running GGD recipe: GRCh37 dbsnp 151-20180423
[2020-05-27T07:00Z] DEBUG: Traceback (most recent call last):
[2020-05-27T07:00Z] DEBUG: File "/usr/local/bin/bcbio_nextgen.py", line 228, in <module>
[2020-05-27T07:00Z] DEBUG: install.upgrade_bcbio(kwargs["args"])
[2020-05-27T07:00Z] DEBUG: File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 107, in upgrade_bcbio
[2020-05-27T07:00Z] DEBUG: upgrade_bcbio_data(args, REMOTES)
[2020-05-27T07:00Z] DEBUG: File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 377, in upgrade_bcbio_data
[2020-05-27T07:00Z] DEBUG: args.cores, ["ggd", "s3", "raw"])
[2020-05-27T07:00Z] DEBUG: File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 354, in install_data_local
[2020-05-27T07:00Z] DEBUG: _prep_genomes(env, genomes, genome_indexes, ready_approaches, data_filedir)
[2020-05-27T07:00Z] DEBUG: File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 480, in _prep_genomes
[2020-05-27T07:00Z] DEBUG: retrieve_fn(env, manager, gid, idx)
[2020-05-27T07:00Z] DEBUG: File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 875, in _install_with_ggd
[2020-05-27T07:00Z] DEBUG: ggd.install_recipe(os.getcwd(), env.system_install, recipe_file, gid)
[2020-05-27T07:00Z] DEBUG: File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 30, in install_recipe
[2020-05-27T07:00Z] DEBUG: recipe["recipe"]["full"]["recipe_type"], system_install)
[2020-05-27T07:00Z] DEBUG: File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 62, in _run_recipe
[2020-05-27T07:00Z] DEBUG: subprocess.check_output(["bash", run_file])
[2020-05-27T07:00Z] DEBUG: File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 356, in check_output
[2020-05-27T07:00Z] DEBUG: **kwargs).stdout
[2020-05-27T07:00Z] DEBUG: File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 438, in run
[2020-05-27T07:00Z] DEBUG: output=stdout, stderr=stderr)
[2020-05-27T07:00Z] DEBUG: subprocess.CalledProcessError: Command '['bash', '/mnt/biodata/genomes/Hsapiens/GRCh37/txtmp/ggd-run.sh']' returned non-zero exit status 1.
[2020-05-27T07:00Z] ERROR: Uncaught exception occurred
Traceback (most recent call last):
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbio/provenance/do.py", line 26, in run
_do_run(cmd, checks, log_stdout, env=env)
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbio/provenance/do.py", line 106, in _do_run
raise subprocess.CalledProcessError(exitcode, error_msg)
subprocess.CalledProcessError: Command 'docker attach --no-stdin 12d33b4f2f096c192aad42a773fdad0abe0b6d86e71ec75b057939918ec41691
--2020-05-27 06:45:28-- https://github.com/chapmanb/cloudbiolinux/archive/master.tar.gz
Resolving github.com (github.com)... 13.234.210.38
Connecting to github.com (github.com)|13.234.210.38|:443... connected.
HTTP request sent, awaiting response... 302 Found
Location: https://codeload.github.com/chapmanb/cloudbiolinux/tar.gz/master [following]
--2020-05-27 06:45:28-- https://codeload.github.com/chapmanb/cloudbiolinux/tar.gz/master
Resolving codeload.github.com (codeload.github.com)... 13.127.152.42
Connecting to codeload.github.com (codeload.github.com)|13.127.152.42|:443... connected.
HTTP request sent, awaiting response... 200 OK
Length: unspecified [application/x-gzip]
Saving to: 'STDOUT'
0K ........ ........ ........ ........ ........ ........ 6.95M
3072K ........ ........ ........ ....... 7.06M=0.7s
2020-05-27 06:45:30 (6.99 MB/s) - written to stdout [5189878]
--2020-05-27 06:45:30-- http://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
Resolving ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)... 130.14.250.7, 2607:f220:41e:250::11
Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:80... connected.
HTTP request sent, awaiting response... 301 Moved Permanently
Location: https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz [following]
--2020-05-27 06:45:35-- https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:443... connected.
HTTP request sent, awaiting response... 416 Requested Range Not Satisfiable
The file is already fully retrieved; nothing to do.
[W::bgzf_read_block] EOF marker is absent. The input is probably truncated
tbx_index_build failed: variation/dbsnp-151.vcf.gz
Upgrading bcbio
Upgrading bcbio-nextgen data files
List of genomes to get (from the config file at '{'genomes': [{'dbkey': 'GRCh37', 'name': 'Human (GRCh37)', 'indexes': ['seq', 'twobit'], 'annotations': ['GA4GH_problem_regions', 'capture_regions', 'MIG', 'prioritize', 'dbsnp', 'hapmap', '1000g_omni_snps', 'ACMG56_genes', '1000g_snps', 'mills_indels', 'clinvar', 'cosmic', 'ancestral', 'qsignature', 'genesplicer', 'effects_transcripts', 'varpon', 'vcfanno', 'viral', 'transcripts', 'RADAR', 'fusion-blacklist', 'mirbase'], 'validation': ['giab-NA12878', 'giab-NA24385', 'giab-NA24631', 'dream-syn3', 'dream-syn4', 'giab-NA12878-NA24385-somatic', 'giab-NA24143', 'giab-NA24149', 'giab-NA24694', 'giab-NA24695']}], 'genome_indexes': ['bwa', 'rtg'], 'install_liftover': False, 'install_uniref': False}'): Human (GRCh37)
Running GGD recipe: GRCh37 dbsnp 151-20180423
Traceback (most recent call last):
File "/usr/local/bin/bcbio_nextgen.py", line 228, in <module>
install.upgrade_bcbio(kwargs["args"])
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 107, in upgrade_bcbio
upgrade_bcbio_data(args, REMOTES)
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 377, in upgrade_bcbio_data
args.cores, ["ggd", "s3", "raw"])
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 354, in install_data_local
_prep_genomes(env, genomes, genome_indexes, ready_approaches, data_filedir)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 480, in _prep_genomes
retrieve_fn(env, manager, gid, idx)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 875, in _install_with_ggd
ggd.install_recipe(os.getcwd(), env.system_install, recipe_file, gid)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 30, in install_recipe
recipe["recipe"]["full"]["recipe_type"], system_install)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 62, in _run_recipe
subprocess.check_output(["bash", run_file])
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 356, in check_output
**kwargs).stdout
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 438, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['bash', '/mnt/biodata/genomes/Hsapiens/GRCh37/txtmp/ggd-run.sh']' returned non-zero exit status 1.
' returned non-zero exit status 1.
Stopping docker container
Traceback (most recent call last):
File "/usr/local/bin/bcbio_vm.py", line 354, in <module>
args.func(args)
File "/usr/local/bin/bcbio_vm.py", line 40, in cmd_install
install.full(args, devel.DOCKER)
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbiovm/docker/install.py", line 40, in full
manage.run_bcbio_cmd(args.image, dmounts, _get_cl(args))
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbiovm/docker/manage.py", line 48, in run_bcbio_cmd
raise e
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbiovm/docker/manage.py", line 44, in run_bcbio_cmd
log_stdout=True)
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbio/provenance/do.py", line 26, in run
_do_run(cmd, checks, log_stdout, env=env)
File "/home/zuser/install/bcbio-vm/anaconda/lib/python3.7/site-packages/bcbio/provenance/do.py", line 106, in _do_run
raise subprocess.CalledProcessError(exitcode, error_msg)
subprocess.CalledProcessError: Command 'docker attach --no-stdin 12d33b4f2f096c192aad42a773fdad0abe0b6d86e71ec75b057939918ec41691
--2020-05-27 06:45:28-- https://github.com/chapmanb/cloudbiolinux/archive/master.tar.gz
Resolving github.com (github.com)... 13.234.210.38
Connecting to github.com (github.com)|13.234.210.38|:443... connected.
HTTP request sent, awaiting response... 302 Found
Location: https://codeload.github.com/chapmanb/cloudbiolinux/tar.gz/master [following]
--2020-05-27 06:45:28-- https://codeload.github.com/chapmanb/cloudbiolinux/tar.gz/master
Resolving codeload.github.com (codeload.github.com)... 13.127.152.42
Connecting to codeload.github.com (codeload.github.com)|13.127.152.42|:443... connected.
HTTP request sent, awaiting response... 200 OK
Length: unspecified [application/x-gzip]
Saving to: 'STDOUT'
0K ........ ........ ........ ........ ........ ........ 6.95M
3072K ........ ........ ........ ....... 7.06M=0.7s
2020-05-27 06:45:30 (6.99 MB/s) - written to stdout [5189878]
--2020-05-27 06:45:30-- http://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
Resolving ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)... 130.14.250.7, 2607:f220:41e:250::11
Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:80... connected.
HTTP request sent, awaiting response... 301 Moved Permanently
Location: https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz [following]
--2020-05-27 06:45:35-- https://ftp.ncbi.nih.gov/snp/pre_build152/organisms/human_9606_b151_GRCh37p13/VCF/GATK/All_20180423.vcf.gz
Connecting to ftp.ncbi.nih.gov (ftp.ncbi.nih.gov)|130.14.250.7|:443... connected.
HTTP request sent, awaiting response... 416 Requested Range Not Satisfiable
The file is already fully retrieved; nothing to do.
[W::bgzf_read_block] EOF marker is absent. The input is probably truncated
tbx_index_build failed: variation/dbsnp-151.vcf.gz
Upgrading bcbio
Upgrading bcbio-nextgen data files
List of genomes to get (from the config file at '{'genomes': [{'dbkey': 'GRCh37', 'name': 'Human (GRCh37)', 'indexes': ['seq', 'twobit'], 'annotations': ['GA4GH_problem_regions', 'capture_regions', 'MIG', 'prioritize', 'dbsnp', 'hapmap', '1000g_omni_snps', 'ACMG56_genes', '1000g_snps', 'mills_indels', 'clinvar', 'cosmic', 'ancestral', 'qsignature', 'genesplicer', 'effects_transcripts', 'varpon', 'vcfanno', 'viral', 'transcripts', 'RADAR', 'fusion-blacklist', 'mirbase'], 'validation': ['giab-NA12878', 'giab-NA24385', 'giab-NA24631', 'dream-syn3', 'dream-syn4', 'giab-NA12878-NA24385-somatic', 'giab-NA24143', 'giab-NA24149', 'giab-NA24694', 'giab-NA24695']}], 'genome_indexes': ['bwa', 'rtg'], 'install_liftover': False, 'install_uniref': False}'): Human (GRCh37)
Running GGD recipe: GRCh37 dbsnp 151-20180423
Traceback (most recent call last):
File "/usr/local/bin/bcbio_nextgen.py", line 228, in <module>
install.upgrade_bcbio(kwargs["args"])
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 107, in upgrade_bcbio
upgrade_bcbio_data(args, REMOTES)
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/site-packages/bcbio/install.py", line 377, in upgrade_bcbio_data
args.cores, ["ggd", "s3", "raw"])
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 354, in install_data_local
_prep_genomes(env, genomes, genome_indexes, ready_approaches, data_filedir)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 480, in _prep_genomes
retrieve_fn(env, manager, gid, idx)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/genomes.py", line 875, in _install_with_ggd
ggd.install_recipe(os.getcwd(), env.system_install, recipe_file, gid)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 30, in install_recipe
recipe["recipe"]["full"]["recipe_type"], system_install)
File "/home/zuser/tmpbcbio-install/cloudbiolinux/cloudbio/biodata/ggd.py", line 62, in _run_recipe
subprocess.check_output(["bash", run_file])
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 356, in check_output
**kwargs).stdout
File "/usr/local/share/bcbio-nextgen/anaconda/lib/python3.6/subprocess.py", line 438, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['bash', '/mnt/biodata/genomes/Hsapiens/GRCh37/txtmp/ggd-run.sh']' returned non-zero exit status 1.
' returned non-zero exit status 1.
Could anyone help me out?
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