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@@ -55,10 +55,11 @@ To deactivate the environment: ```$> deactivate``` | |
<a name="demo"></a> | ||
### Tutorial | ||
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We recommend trying the provided [demo notebook](notebooks/tutorial.ipynb) to ensure that the software was properly | ||
installed and to experiment running Cue. In this demo we will use Cue to discover variants in | ||
a small BAM file provided here: ```data/demo/inputs/chr21.small.bam``` (with the associated YAML config files needed | ||
to execute this workflow provided in the ```data/demo/config``` directory). | ||
We recommend trying the provided demo Jupyter [notebook](notebooks/tutorial.ipynb) to ensure that the software | ||
was properly installed and to experiment running Cue. For convenience, Jupyter was already included in the installation | ||
requirements above, or can be installed separately from [here](http://jupyter.org/install). | ||
In this demo we use Cue to discover variants in a small BAM file provided here: ```data/demo/inputs/chr21.small.bam``` | ||
(with the associated YAML config files needed to execute this workflow provided in the ```data/demo/config``` directory). | ||
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<a name="guide"></a> | ||
### User guide | ||
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```view.py```: | ||
* ```bam``` [*required*] path to the alignments BAM file | ||
* ```bed``` [*required*] path to the BED or VCF file with SVs to visualize | ||
* ```fai``` [*required*] path to the referene FASTA FAI file | ||
* ```fai``` [*required*] path to the reference FASTA FAI file | ||
* ```n_cpus``` [*optional*] number of CPUs (parallelized by chromosome) | ||
* ```chr_names``` [*optional*] list of chromosomes to process: null (all) or a specific list e.g. ["chr1", "chr21"] | ||
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3. Populate the YAML config file(s) with the parameters specific to this experiment. | ||
4. Execute the appropriate ```engine``` script providing the path to the newly configured YAML file(s). | ||
The engine scripts will automatically create auxiliary directories with results in the folder where the config YAML files are located. | ||
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#### Authors | ||
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Victoria Popic ([email protected]) | ||
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#### Feedback and technical support | ||
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For questions, suggestions, or technical assistance, please create an issue on the Cue | ||
[Github issues](https://github.com/PopicLab/cue/issues) page or | ||
reach out to Victoria Popic at [email protected]. |