Updated the Ribo ITP Website

Changed the Reference in Purpose
Changed 2 of the steps in Pre-experimental set-up
Changed Manufacturing RIbo-ITP Chips section
Changed Ribosome profiling via ITP section

_tabs/ribo_itp.html

@@ -68,7 +68,7 @@ <h2>
   <ol type="A">
 
     <li class="subpheader" >Purpose</li>
-    The purpose of this protocol is to detail the RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP) protocol described in Tonn et al. 2021 to maximize execution and reproducibility in other laboratories. Here, we detail all reagents and materials needed, steps to take for experimental setup, and the Ribo-ITP protocol, and general considerations. 
+    The purpose of this protocol is to detail the RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP) protocol described in Ozadam et al. 2023 to maximize execution and reproducibility in other laboratories. Here, we detail all reagents and materials needed, steps to take for experimental setup, and the Ribo-ITP protocol, and general considerations. 
   </ol>
 
   <!-- OVERVIEW ENDS  -->
@@ -134,7 +134,7 @@ <h2>
        <tr>
          <td>BisTris-HCl Master Mix </td>
          <td>Buffer Preparation  </td>
-         <td>130mM BisTris and 20mM HCl in a solution with Vf=1  </td>
+         <td>2.35M BisTris and 361mM HCl in a solution  </td>
          <td>2.5M BisTris- 4940 uL, 6M HCl- 316.35 uL</td>
        </tr>
        <tr>
@@ -147,7 +147,7 @@ <h2>
          <td>Lysis Buffer Stock  </td>
          <td>Sample Preparation </td>
          <td>20 mM BisTris, 1% Triton X-100, 5 mM MgCl2, 5 mM MgCl2, 100 mM NaCl, 4.4 mM HCl  </td>
-         <td>1M BisTris titrated to 7.2- 600 uL, 1M CaCl2- 150 uL, 10% Triton x-100- 3 mL, 1M MgCl2- 150 uL, 5M NaCl- 600 uL, NFH2O- 25.5 mL </td>
+         <td>1M BisTris titrated to 7.2- 600 uL, 5 mM CaCl2- 150 uL, 10% Triton x-100- 3 mL, 1M MgCl2- 150 uL, 5M NaCl- 600 uL, NFH2O- 25.5 mL </td>
        </tr>
        <tr>
          <td>  Storage Buffer </td>
@@ -355,7 +355,7 @@ <h2>
 	   <br>
 	   <br>
 	   <li> Turn on the RF power to “HI” and treat the samples for 2 min. Bright violet plasma color should be observed.  </li>
-	   <li> Turn off the RF and vacuum pump, and close the 2-way valve (lever pointing down).  </li>
+	   <li> Turn off the RF and vacuum pump, and close the 3-way valve (lever pointing down).  </li>
 	   <li> Slowly vent the chamber by opening the 3-way valve slightly (lever pointing slightly to the right).  </li>
 	   <li> Should hear a hissing sound as the air evacuates. This should be done slowly (takes at least 1 min).  </li>
 	   <li> Chamber is fully vented when pressure returns to atmospheric pressure  </li>
@@ -571,7 +571,7 @@ <h2>
     <li class="subpheader" > RIBOsome profiling via IsoTachoPhoresis (Ribo-ITP)  </li>
     <ol type="1">
         <li>Cover the BlueLight source (Clare Chemical Dark Reader Transilluminator) with its light filter then turn it on to enable fluorophore visualization. Turn off lights in order to visualize fluorophore well.  </li>
-        <li> A Keithley 2410 Sourcemeter, controlled using MatLab was used to apply a constant 300 mA across the channel with a maximum voltage of 1.1 kV.  </li>
+        <li> A Keithley 2410 Sourcemeter, controlled using MatLab was used to apply a constant 300 uA across the channel with a maximum voltage of 1.1 kV.  </li>
         <li> Apply current to the channel to start sample migration.  </li>
         <li> When the fluorescent band passes by Branch Channel 1, a secondary negative lead was added to LE Reservoir 1.  </li>
 	<ol type="a">