Updated the Buffer Preparation Section

Lysis Buffer Working Solution

_tabs/ribo_itp.html

@@ -143,6 +143,12 @@ <h2>
          <td>1M BisTris and 220mM HCl </td>
          <td>BisTris- 10.462 g, NF-H2O- 30 mL, 6M HCl- 1820 uL, NF-H2O- To final volume of 50 mL, </td>
        </tr>
+       <tr>
+        <td>Lysis Buffer Working Solution  </td>
+        <td>Buffer Preparation  </td>
+        <td>20 mM Bis-Tris, 1.0% Triton-X 100, 5 mM MgCl2, 5 mM CaCl2, 100 mM NaCl, 1 mM DTT, 0.1 mg/mL cycloheximide</td>
+        <td>Add 989 µL Lysis Buffer Stock, 10 µL DTT [100 mM], and 1 µL cycloheximide [100 mg/mL]. Keep on ice.</td>
+       </tr>
        <tr>
          <td>Lysis Buffer Stock  </td>
          <td>Sample Preparation </td>