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vignettes/images/DEGcounts.png

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vignettes/images/sample_tree.png

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vignettes/images/vennplot.png

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vignettes/systemPipeR.Rmd

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dependency = "preprocessing")
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```
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<center><img src="results/fastqReport.png"></center>
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<center><img src="images/fastqReport.png"></center>
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<div align="center">**Figure 1:** FASTQ quality report </div></br>
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## NGS Alignment software
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dependency = "read_counting")
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```
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<center><img src="results/sample_tree.png"></center>
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<center><img src="images/sample_tree.png"></center>
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<div align="center">**Figure 2:** Correlation dendrogram of samples for _`rlog`_ values. </div></br>
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Filter and plot DEG results for up and down-regulated genes. Because of the small size of the toy data set used by this vignette, the _FDR_ value has been set to a relatively high threshold (here 10%). More commonly used _FDR_ cutoffs are 1% or 5%. The definition of '_up_' and '_down_' is given in the corresponding help file. To open it, type _`?filterDEGs`_ in the R console.
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<center><img src="results/DEGcounts.png"></center>
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<center><img src="images/DEGcounts.png"></center>
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<div align="center">**Figure 3:** Up and down regulated DEGs identified by _`edgeR`_. </div></br>
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dependency = "edger")
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```
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<center><img src="results/vennplot.png"></center>
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<center><img src="images/vennplot.png"></center>
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<div align="center">**Figure 4:** Venn Diagram for 4 Up and Down DEG Sets. </div></br>
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## GO term enrichment analysis of DEGs
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The _`data.frame`_ generated by _`GOCluster_Report`_ can be plotted with the _`goBarplot`_ function. Because of the variable size of the sample sets, it may not always be desirable to show the results from different DEG sets in the same bar plot. Plotting single sample sets is achieved by subsetting the input data frame as shown in the first line of the following example.
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<center><img src="results/GOslimbarplotMF.png"></center>
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<center><img src="images/GOslimbarplotMF.png"></center>
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<div align="center">**Figure 5:** GO Slim Barplot for MF Ontology.</div></br>
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```
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<center><img src="results/heatmap1.png"></center>
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<center><img src="images/heatmap1.png"></center>
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<div align="center">**Figure 7:** Heat map with hierarchical clustering dendrograms of DEGs.</div></br>
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## Visualize workflow

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