How can I load sequences from a CSV file and do the massive Blast search locally?

[Ecotone23]

Hi,

I have a CSV dataset of edited DNA sequences by DADA2 pipeline and wonder how I can load and blast these sequences automatically using metablastr packages.

In the CSV file, each row represents a unique sequence and each column has a sample name(see attached image):

These COI gene sequences are clean and ready to Blast directly on NCBI website. Most of the sequences are from mammalian and avian blood. Since there are over 2000 sequences, it'd be great if I can use this package to load and blast automatically instead of manually.

Any R scripts to achieve this goal with metablastr package would highly appreciated. Thank you.

Best,

Gabriel