hicPCA flip signs with rna-seq?

[Naveen-Ahuja]

Hi,

I would like to know if it makes sense to use rnaseq bigwig file in hicpca to flip the signs. For example, choose extra track type --> Histone Mark --> Histone Mark type=active. Would this be appropriate as there isn't an explicit rnaseq option, only histone mark and gene density?

Thank you

[Naveen-Ahuja]

Just following up using a bigwig file gives this error on the hicexplorer web:

`Fatal error: Exit code 1 ()
Tool generated the following standard error:

Traceback (most recent call last):
File "/usr/local/tools/_conda/envs/[email protected]/bin/hicPCA", line 7, in
main()
File "/usr/local/tools/_conda/envs/[email protected]/lib/python3.8/site-packages/hicexplorer/hicPCA.py", line 337, in main
vecs_list = correlateEigenvectorWithGeneTrack(ma, vecs_list, args.extraTrack)
File "/usr/local/tools/_conda/envs/[email protected]/lib/python3.8/site-packages/hicexplorer/hicPCA.py", line 145, in correlateEigenvectorWithGeneTrack
bed = ReadBed(file_h)
File "/usr/local/tools/_conda/envs/[email protected]/lib/python3.8/site-packages/hicexplorer/readBed.py", line 35, in init
fields = self.get_no_comment_line()
File "/usr/local/tools/_conda/envs/[email protected]/lib/python3.8/site-packages/hicexplorer/readBed.py", line 69, in get_no_comment_line
line = toString(line)
File "/usr/local/tools/_conda/envs/[email protected]/lib/python3.8/site-packages/hicexplorer/utilities.py", line 539, in toString
return s.decode('ascii')
UnicodeDecodeError: 'ascii' codec can't decode byte 0xfc in position 1: ordinal not in range(128)`