Update README.md

Author: ezrabru

README.md

@@ -41,5 +41,3 @@ The estimation of the offset, variance and gain was implemented as described in
 **Tip 3:** If you don't have a brightfield lamp on your microscope with adjustable brightness, you can use another lamp and layer lens tissues to decrease the intensity in steps. You can also use your laser as a light source if you have a fluorescent slide (e.g. from [Chroma](https://www.chroma.com/products/accessories/92001-autofluorescent-plastic-slides)) instead of a piece of paper. The latter works best when your illumination profile is flat over your roi.
 
 **Tip 4:** The brightness of your lamp should be constant during each stack. If your lamp is unstable or has a fluctuating brightness, this will mess up the calibration. [Diekmann *et al.*](https://doi.org/10.1038/s41598-017-14762-6) used a TN-LED laptop screen as a light source.
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-**Tip 5:** If your images don't have a uniform intensity, the pixel-dependent offset, variance and gain maps will still be accurate, but the average offset, variance and gain probably won't be. Getting uniform intensity for small regions (e.g. 512x512 pixels) is not hard, but it can be more tricky when you want to calibrate a large sCMOS chip in one go while it's attached to a microscope.