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Reads with identical UMIs and mapping coordinates output by Gencore #45

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luederm opened this issue Jun 22, 2022 · 0 comments
Open

Reads with identical UMIs and mapping coordinates output by Gencore #45

luederm opened this issue Jun 22, 2022 · 0 comments

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@luederm
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luederm commented Jun 22, 2022 •
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Hello,

I am running the latest gencore with --no_duplex and -s = 3. However, I am still getting many reads output which have the same UMI and identical mapping coordinates. Please see the IGV screenshot below. Reads are shown as pairs and are grouped by UMI, which can be seen on the left.

image
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@luederm