Skip to content

Coordinate X and Y values #51

@mortunco

Description

@mortunco

Hello,

I apologize for my ignorance if this is very obvious.

This is also a cross post from https://www.biostars.org/p/9566492/

Basically, what is the unit of the X,Y from the puck data?

I see The final stitched images were 6030 pixels by 6030 pixels. from the 2019 paper (v1) and 2021 paper (v2).

I am little bit confused because I have seen negative X, Y and also float values.

$head Puck_180413_7.idx
cellid,index,x,y
c1,GAACTAAAAACCC,2242.48275862069,1349.89655172414
c2,AGCTGTAAAGGGG,1707.53731343284,3820.50746268657
c3,TCTTCTAGAAGGT,1166.06842105263,4450.2
c4,TGCCAGCAAAGGA,4281.8679245283,4462.67924528302

Any guidance would be super valuable. Especially, regarding how to deal with batches coming from different laboratories/experiments.

Best regards,

Tunc.

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions