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Hello,
I apologize for my ignorance if this is very obvious.
This is also a cross post from https://www.biostars.org/p/9566492/
Basically, what is the unit of the X,Y from the puck data?
I see The final stitched images were 6030 pixels by 6030 pixels. from the 2019 paper (v1) and 2021 paper (v2).
I am little bit confused because I have seen negative X, Y and also float values.
$head Puck_180413_7.idx
cellid,index,x,y
c1,GAACTAAAAACCC,2242.48275862069,1349.89655172414
c2,AGCTGTAAAGGGG,1707.53731343284,3820.50746268657
c3,TCTTCTAGAAGGT,1166.06842105263,4450.2
c4,TGCCAGCAAAGGA,4281.8679245283,4462.67924528302
Any guidance would be super valuable. Especially, regarding how to deal with batches coming from different laboratories/experiments.
Best regards,
Tunc.
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