Add Nuclei Resuspension Reagent to Library Preparation Schema

[rachadele]

For which schema is a change/update being suggested?

would like to request an update to the library_preparation_protocol.json schema.

What should the change/update be?

I would like to add a new field - nuclei resuspension reagent - to this schema to allow data contributors to describe the reagent used to resuspend nuclei for snRNA-seq.

This update constitutes a minor change to the schema(s) it affects.

What new field(s) need to be changed/added?

• Field name: nuclei_resuspension_reagent
• Field description: A reagent used to resuspend nuclei
• Field type: string
• Required: no
• Examples: 0.1% NP40-PBS, Nuclei EZ Lysis Buffer
• CV or enum: no

Why is the change requested?

Data contributors need to be describe different reagents used to resuspend nuclei in a snRNA-seq experiment