Feasibility of xpore to work on non-m6A modification

[YanqiangLi]

Hi, Thanks for your great work for xpore.
May I ask are there feasibility of xpore to work on non-m6A modification with WT and knock down/out ?
Do I need to prepare another model myself?

Thank you!

[ploy-np]

Hi @YanqiangLi,

Yes, you can use xpore to find the differential modification rates between WT and knock down/out samples for any modification types including non-m6As.

[YanqiangLi]

Thanks for your prompt reply.
Yes, I am working on it. From the first step, it said "Using the signal of unmodified RNA from /programs/x86_64-linux/xpore/2.1/lib/python3.10/site-packages/xpore/diffmod/model_kmer.csv" , from my understanding, here the model_kmer.csv is from the non-m6A modifiled signal, should I prepare the signal of unmodified RNA from IVT to do the analysis when I want to do non-m6A analysis?
Thank you!