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More complex barcodes are activated with --soloType CB_UMI_Complex and are described with the following parameters
soloCBposition -
strings(s) position of Cell Barcode(s) on the barcode read.
Presently only works with --soloType CB_UMI_Complex, and barcodes are assumed to be on Read2.
Format for each barcode: startAnchor_startDistance_endAnchor_endDistance
start(end)Anchor defines the anchor base for the CB: 0: read start; 1: read end; 2: adapter start; 3: adapter end
start(end)Distance is the distance from the CB start(end) to the Anchor base
String for different barcodes are separated by space.
Example: inDrop (Zilionis et al, Nat. Protocols, 2017):
--soloCBposition 0_0_2_-1 3_1_3_8
soloUMIposition -
string position of the UMI on the barcode read, same as soloCBposition
Example: inDrop (Zilionis et al, Nat. Protocols, 2017):
--soloUMIposition 3_9_3_14
soloAdapterSequence -
string: adapter sequence to anchor barcodes.
soloAdapterMismatchesNmax 1
int>0: maximum number of mismatches allowed in adapter
also it has options to provide multiple white list coresponding to the barcodes:
--soloCBwhitelist
default: -
string(s): file(s) with whitelist(s) of cell barcodes. Only –soloType CB_UMI_Complex allows more than one whitelist file.
None no whitelist: all cell barcodes are allowed
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More complex barcodes are activated with --soloType CB_UMI_Complex and are described with the following parameters
also it has options to provide multiple white list coresponding to the barcodes:
The text was updated successfully, but these errors were encountered: